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Efflux Simulation Software
Efflux Simulation Software
Advanced in-silico chromatography simulation platform
Efflux Contract Modeling
Efflux Contract ModelingNew
You perform the experiments, we do the modeling
Buffer Calculator
Calculate pH buffer recipes
Column Characterization
Calculate column parameters
Extinction Coefficient
Estimate extinction coefficients
Flowrate ConversionNew
Convert flow rates
Protein ParametersNew
Protein properties from sequence
UNICORN Data ExtractorNew
Extract data from UNICORN files

About Us

We help bioprocess teams accelerate development and reduce experimental costs by providing chromatography simulation software and services.

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Fundamentals

Getting StartedExperimental DataInterpreting ResultsBest PracticesFurther Reading

Scientific Background

Mechanistic ModelingUnit OperationsColumn ModelsBinding ModelsSimulation

Guides

How to characterize a columnHow to calibrate a modelHow to optimize yield and purityHow to perform a process parameter screening
Getting Started
Experimental Data
Interpreting Results
Best Practices
Further Reading
Mechanistic Modeling
Unit Operations
Column Models
Binding Models
Simulation
How to characterize a column
How to calibrate a model
How to optimize yield and purity
How to perform a process parameter screening
  1. Learn
  2. Guides

Guides

Practical, step-by-step guides for common Efflux workflows — from setting up unit operations to optimizing separation conditions.

How to Characterize a Column

Determine column parameters like porosity, axial dispersion, and mass transfer coefficients from pulse injection experiments.

How to Calibrate a Model

Fit binding and transport parameters to experimental data for accurate mechanistic model predictions.

How to Optimize Yield and Purity

Use simulation to explore gradient profiles, flow rates, and loading conditions to maximize separation performance.

How to Perform a Process Parameter Screening

Systematically vary process parameters to map the design space and identify robust operating conditions.

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