Chromatography Simulations For Process Developers

Secure cloud-based chromatography modeling, right in your browser

Shorten time to milestones

Lower cost and risk

Improve process understanding

Interactive Demo

Proteins

Elution

This is an interactive demo. Full version offers complete control over all process parameters, system settings, column properties, and much more. Learn more

How It Works

Create the Model

Define your columns, chromatography system, and experimental data.

Define your columns

Select among predefined templates, or define your own custom columns

Calibrate the Model

Fit model parameters to experimental data. Typically, 5-8 experiments are needed to build a well-fitted model. The calibration usually takes a few hours, so be patient.

Validate the Model

Validate the model accuracy by performing an additional 1-3 experiments, and compare these unseen experiments with the simulation results. If the model fits, you are good to go! If not, repeat the calibration step with the new experiments included.

Use the Model

Once you have validated your model, use it to simulate thousands of experiments in minutes.

Why Mechanistic Modeling?

Imagine being able to simulate any chromatography experiment you want. That is what mechanistic modeling enables. It is a digital twin for your entire downstream process lifecycle.

Speak with an expert

Fewer experiments

Reduce the number of experiments and thus time and cost compared to traditional process development methodologies.

Increase process understanding

Provides full insight into process parameters, correlations, parameter sensitivies, and much more.

Quality by Design by Design

All of Efflux's modeling solutions are built to systematically promote, achieve, and follow Quality By Design (QbD) principles.

Common Questions

Mechanistic modeling uses physics-based equations to simulate chromatography experiments. Physical mechanisms such as convection, diffusion, mass transfer, and adsorption are described using first-principles equations. By using mechanistic modeling, the number of real-world experiments can be reduced compared to statistical modeling approaches, while greatly improving process understanding.

Mechanistic modeling is based on physical equations that describe the underlying mechanisms within the chromatography column. Statistical models (like DoE) are based on correlations between inputs and outputs and cannot explain the underlying process mechanisms. The advantage of mechanistic models is that they typically require fewer experiments to optimize process parameters, while simultaneously providing a better process understanding.

With the Efflux modeling platform, anyone can simulate chromatography experiments directly in the browser. We have tailored Efflux for downstream process development, scale-up, screening, root-cause analysis, and tech-transfers. However, Efflux is able to simulate all types of chromatography experiments.

Yes. We understand that your data is sensitive. We use state-of-the-art security systems to keep all of your sensitive data secure. Very sensitive data, like protein sequences, are stored encrypted, meaning that even in the unlikely event of data leakage, your sequences remain secure. Learn more about our security practices.

Yes, we offer contract modeling services. Partner up with us and let our modeling experts handle the modeling for you. That way, you can focus on what you do best and still save time and resources during process development. Learn more about our contract modeling service.